Fig. 1
From: Whi7 is an unstable cell-cycle repressor of the Start transcriptional program
Cell cycle regulation of Whi7. cdc15 cells expressing GFP-tagged Whi7, HA-tagged Clb2, and myc-tagged Sic1 (JCY1802) were arrested in telophase by incubation at 37 °C. After 3 h, cells were transferred to 25 °C and cell cycle progression was analyzed at the indicated time. a Graph shows the distribution of cells at different cell cycle stages. b Whi7, Clb2, and Sic1 proteins, as well as Cdc28 as loading control, were analyzed by western blot; left graph shows the relative amount of each protein referred to the most abundant sample; right graph represents the percent of total Whi7 protein present as slow migrating bands. MCY123 and no tagged cdc15 strains were included in western analysis as controls
