Fig. 2

Variation in 21U piRNAs underlies nictation differences. a Genotypes of near isogenic lines (NILs) represented by orange (N2) and blue (CB4856) colored bars are shown. Strain labels are to the right. Solid vertical lines denote the confidence interval from the genetic mapping experiment. Dotted lines denote the narrowed genetic interval. b The mean nictation fractions of N2 (orange), CB4856 (blue), and NILs are shown as horizontal bars. Error bars are standard deviations. The number of biological replicates for each strain range from 17 to 49. c Genomic features of NIL-narrowed 73 kb nict-1 QTL interval are shown. Protein-coding genes (top) are colored orange (higher expression in the N2 nict-1 strain), blue (higher expression in CB4856 nict-1 strain), or gray (no expression difference between the nict-1 genotypes). The blue dot denotes a predicted functional effect in Y105C5A.1272. The locations of piRNAs and other genomics features (tRNAs, snoRNAs, etc.) are shown in gray in the middle and bottom rows. d Tukey box plots of normalized nictation fractions of N2 (orange), LJ1203 (nict-1 CB4856 > N2, blue), ECA586 (prg-1(ean28) in the N2 genetic background, gray), CB4856 (blue), LJ1213 (nict-1 N2 > CB4856, orange), ECA584 (prg-1(ean30) in the CB4856 genetic background, gray) are shown with data points plotted behind. The horizontal line is the median, and the box denotes the 25th to 75th quantiles of the data. The vertical line represents the 1.5 interquartile range. All strains were scored in 21 biological replicates, except for strain ECA584 where 19 biological replicates were scored. Each biological replicate was composed of three technical replicates