Fig. 3
From: Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis
Ubiquitination of RIPK1 is essential for its phosphorylation by TAK1. a SM-164 treatment blocks RIPK1 S321 phosphorylation in L929 cells. The cells were pre-treated by SM-164 (50 nM) and/or 5Z-7 (0.5 µM) as indicated for 30 min, and incubated with TNFα (10 ng/ml) for 5 min. b RIPK1 S321 is not phosphorylated in the absence of TRAF2, TRADD or cIAP1/2. TRAF2, TRADD, cIAP1/2 KO MEFs and their corresponding WT MEFs were treated with TNFα for 5 min. c RIPK1 S321 hyperphosphorylation in A20 KO MEFs. A20 KO and WT MEFs were treated with TNFα and samples were collected at indicated time-points. The phosphorylation of RIPK1 S321 was analyzed by western blot using indicated antibodies a–c
