Fig. 2

Establishing a panel of Spinach-based aptamers that can detect analytes in droplets. A panel of Spinach-based aptamer sensors was tested across a variety of small molecule and protein targets. Aptamers were synthesized through in vitro transcription and incubated with or without the analyte of interest, and fluorescence was measured. Bar graphs represent the mean of three replicates (error bars depict s.d.). On the right, solutions of dye with RNA aptamer or with both RNA aptamer and analyte were encapsulated and the resulting microdroplets were imaged using fluorescence microscopy. The negative (RNA alone) and positive (RNA and analyte) droplets were mixed together for simultaneous imaging of contrast. All images were acquired under the same magnification, and representative images from multiple independent trial runs are depicted. Sequences of all aptamers used in this study can be found in Supplementary Table 1