Fig. 6
Flow-evoked vasoconstriction in mesenteric artery. Isobaric external diameter recordings from second-order mesenteric artery. a Example images of a cannulated artery before and after luminal pressure difference (ΔP) and then after 10 μM nicardipine was added to the recording chamber. Control genotype mouse. Scale bar, 200 μm. b Example diameter recording for a control genotype mouse during incremental increases in ΔP as indicated by the black dots (20, 40, 60, 70, 80, 90 and 100 mm Hg). Nicardipine (Nic, 10 μM), phenylephrine (PE, 1 μM) and acetylcholine (ACh, 10 μM) were applied as indicated. The first arrow indicates addition of 100 μM N(ω)-nitro-L-arginine methyl ester (L-NAME) to the recording chamber and the second arrow multiple washes of the chamber to remove nicardipine and L-NAME. c Mean data for the type of experiment shown in b presented as the constriction to ΔP as a percentage of the PE response (9 arteries from n = 6 mice). Nicardipine significantly (***) reduced the 100 mm Hg ΔP response to 30.7 ± 3.9% (n = 6 mice). d, e The same as for b, c but using Piezo1ΔEC genotype mice (8 arteries from n = 3 mice). Averaged data are displayed as mean ± s.e.m. Data sets are compared by t-test. Statistical significance is indicated by **P < 0.01, ***P < 0.001
