Fig. 7
Proteasome does not regulate p53 protein synthesis in B cells. a Quantitation by flow cytometry of p53 protein expression in LPS-activated B cells treated with MG132 (10 μM), etoposide (20 μM) or actinomycin D (ActD, 5 μg ml−1). Data are from six independent experiments with 2 to 4 biological replicates in each. Mann–Whitney test was performed. b Immunoblot analysis of pSer15-p53, p53 and β-actin in activated B cells treated with Lactacystin (Lact, 1 μM), Nutlin-3 (Nut-3, 10 μM), p53 Activator III RITA (1 μM), SJ172550 (SJ, 1 μM), HBX41108 (HBX, 10 μM) or P005091 (P0, 10 μM) prior induction of DNA damage with etoposide. One of the two independent experiments performed is shown. c Quantization by flow cytometry of p53 expression in B cells treated with different doses of the inhibitors described in b. Data are from six biological replicates collected in two independent experiments. Two-way ANOVA and Bonferroni post-test analysis was performed (ns non-significant, *P < 0.05, ***P < 0.001, ****P < 0.0001)
