Fig. 7
Real-time visualization of DNA methylation levels on major satellite repeats with BiAD sensor 1. a Live-cell imaging of cells stably expressing the BiAD sensor 1 modules. The time points at which images were taken are annotated. The panels are centered on the cell that undergoes mitosis during the imaging interval. To facilitate visualization, this cell was marked with a dotted circle. The imaging and data display conditions are identical between the shown time points. b Representative phase contrast-fluorescence channel overlay images during the 5-aza-dC DNA demethylation treatment of the cell line stably expressing BiAD sensor 1. To facilitate visualization, the BiFC signal was pseudo colored. The imaging and data display conditions are identical between the three panels of the composite image. c Quantification of the experiments representatively shown in panel. Error bars represent s.e.m. for two biological replicates (for details cf. Supplementary Tables 6 and 7). Scale bar for all images is 10 µm
