Fig. 7

Template-assisted product formation in reactions initiated with N and each of the non-native electrophile thioesters (E ^D, Eγ, and E ^D γ) separately. a HPLC chromatograms comparing product formation at 90 min in template-free and template-seeded reactions initiated with Eγ and N (seeding with i-2 or i-2 ^D γ). b Time-dependent formation of the native product 2 in template-free and template-seeded reactions initiated with Eγ and N. c Bar diagram representing the template effects in reactions initiated with N and each of the non-native electrophile thioesters, separately, and seeded each time with a different template (100 ± 10 μM). Template effects were calculated from the respective product formation data at 15 min for reactions initiated with E ^D, and at 90 min for reactions initiated with Eγ or E ^D γ in template-free (Supplementary Fig. 5) and template-assisted (b of this figure and Supplementary Figs. 10–12) reactions