Fig. 5

Adsorption capacities and identified unique phosphorylation site information. a, b Comparison of adsorption capacities of PNI-co-ATBA_0.2@SiO₂ (black columns) and commercially available TiO₂ (red columns) towards 1pS–4pS (a), or towards 3pS, 3pT and 3pY (b). c Comparison of recovery of PNI-co-ATBA_0.2@SiO₂- (black and gray columns) and TiO₂– (red, orange and yellow columns) based enrichment methods towards 1pS–4pS, obtained from three parallel MS measurements. d, e Percentages of unique phosphorylation sites (d) or distribution proportions (e) for mono-, di-, tri- or not less than tetra-PPs enriched by our material from tryptic digests of HeLa S3 cell lysates. f Comparison of relative ratios of serine, threonine to tyrosine phosphorylation for mono-, di-, tri- or not less than tetra-PPs, identified by the polymeric material from the same cell sample. For panels d, e, f, the number of peptides and phosphorylation sites for 1PP–4PP was calculated from the pool of three analytical repeats