Fig. 6
From: Multiscale cytometry and regulation of 3D cell cultures on a chip
Selective spheroid recovery. a Schematic protocol: the selected agarose droplet is locally heated with a focused infrared laser beam (orange dots). A culture medium flow transports the spheroid out of the chip, where it is collected in a 1.5 mL tube. b Time lapse of spheroid recovery. Orange dots indicate the laser position in the anchor. c Bright field and LIVE/DEAD stained spheroid after laser recovery. d Bright field picture of a spheroid immediately after replating (0 h) and 24 h post-replating in 2D dish. All scale bars are 50 μm
