Fig. 8
From: Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients
Nutrient induces FAs accumulation. a The conversion of 13C-palmitate to 13C-citrate was traced in HEK293T cells cultured in low and high glucose media, and in ECHS1 overexpressing HEK293T cells cultured in high glucose media. b Relative FAs levels in HEK293T cells, ECHS1 knocked down HEK293T cells and ECHS1 knocked down HEK293T cells overexpressing shRNA resistant ECHS1 (ECHS1-r) were detected. All levels were normalized to the total proteins. c The relative butyrate levels in Chang liver cells and ECHS1 knocked down Chang liver cells were determined (n = 4). The butyrate levels in untreated Chang liver cells (25 μM) were set as 100%. d The relative FAs levels in HEK293T cells cultured in low glucose (−), high glucose (+), and high glucose with ECHS1K101R overexpression were determined (n = 3). e The FAs levels in the liver of wild type, Sirt3 −/−, and ECHS1 K/R overexpressing Sirt3 −/− 129/C57BL6 mice were assessed by oil red staining. Four mice of each group were analyzed. Relative FAs levels were determined by comparing intensity of signals, Sirt3 +/+ value was set as 100% arbitrarily (right). f The relative (to time 0) intracellular FAs levels in Chang cells that were maintained in high glucose (25 mM) and low glucose (1 mM) were detected (n = 3) at time points as indicated. For all figures, mean values with SD are reported. NS not significant; *P < 0.05; **P < 0.01; ***P < 0.001
