Fig. 1
From: An allosteric ligand-binding site in the extracellular cap of K2P channels
Inhibition of TREK subfamily channels by TKDC in CHO cells. a Chemical structure of TKDC. b, c Typical whole-cell current traces recorded from CHO cells overexpressing the TREK-1 channel with 10 μM TKDC b or DMSO application c. Currents were elicited by depolarizing voltage steps from a holding potential of −80 mV to + 80 mV in 20 mV increments, followed by stepping down to −60 mV. d Dose-dependent inhibition of TKDC on TREK-1, TREK-2 and TRAAK channels. e The statistics of the half-inhibitory concentrations of TKDC for TREK-1 (n = 7), TREK-2 (n = 7), and TRAAK (n = 8) channels. IC50 values were obtained via dose–response fitting. The Kruskal–Wallis test was used for statistical analysis; ** indicates P < 0.01. The data are shown as the mean ± s.e.m
