Fig. 2
From: An allosteric ligand-binding site in the extracellular cap of K2P channels
Binding mode of TKDC to TREK-1. a Binding site of TKDC in the extracellular cap of the TREK-1 channel. b Detailed view of the interactions between TKDC and the TREK-1 channel. TKDC and the residues involved in binding are shown as sticks. The protein is shown in a cartoon and surface depiction. c Dose-dependent inhibition of TKDC on the WT and mutant TREK-1 channels. d Histograms summarizing the half-inhibitory concentrations for WT (n = 7), L102A (n = 10), Q76A (n = 6) and I80A (n = 3) mutant TREK-1 channels. IC50 values were obtained via dose–response fitting. The Kruskal–Wallis test was used for statistical analysis; * indicates P < 0.05, ** indicates P < 0.01. The data are shown as the mean ± s.e.m
