Fig. 4
From: An allosteric ligand-binding site in the extracellular cap of K2P channels
Inhibition of TREK subfamily channels by 28NH in CHO cells. a Chemical structure of 28NH. b Dose-dependent inhibition of TRAAK by TKDC and 28NH. c The statistics of IC50 values for the inhibition of TREK-1, TREK-2 and TRAAK channels by TKDC and 28NH. IC50 values were obtained via dose–response fitting. The unpaired t-test was used for statistical analysis [t (7) = 1.027 and n = 9 for TREK-1, t (4) = 0.910 and n = 6 for TREK-2, and t (6) = 5.724 and n = 8 for TRAAK]; ** indicates P < 0.01. The numbers in the bars indicate the number of cells studied per condition. The data are shown as the mean ± s.e.m. d Binding model of 28NH to TRAAK. The protein is shown as a cartoon. 28NH and key residues are shown as sticks
