Fig. 1

Intestinal helminth infection triggers mLN lymphangiogenesis. C57BL/6 J mice were infected with Hp and the entire chain of the mLN collected at the indicated time-points. a, b LYVE-1⁺ lymphatic networks in the mLN of naive and 21 dpi infected mice were visualized by deep tissue imaging. Temporal color profiles are used to indicate the LYVE-1⁺ lymphatic network in relation to cortical and paracortical regions. SCS; subcapsular sinus (red), PCR paracortical region (blue). c Quantitation of the mLN area occupied by lymphatics (LYVE-1⁺ LECs) in C57BL/6 mice following Hp infection. Data are pooled from two independent experiments and represent mean ± SEM. Each dot represents an individual animal. d Relative expression of mRNA encoding Prox-1 in whole mLN over the indicated time course. Data is from a single experiment and is representative of ≥3 independent experiments. e, f Vibratome sections of mLNs showing 2D and 3D views of combined immunofluorescence staining for B-cell follicles (B220; green) and lymphatics (LYVE-1; red). Scale bar = 50 μm. Images are taken from a single mouse and are representative of three independent experiments. For all data shown, each independent experiment included n ≥ 2–3 mice/group/time-point. See also Supplementary Movies 1–2 and Supplementary movies 4–5