Table 1 Characterisation of the pyoverdine locus and prediction of the pyoverdine amino acid backbone for natural Pseudomonas isolates

From: Siderophore cheating and cheating resistance shape competition for iron in soil and freshwater Pseudomonas communities

Community ID

Strain ID

Strain classification

Pyoverdine production (%)a

Number of genes

Predicted pyoverdine peptide sequenceb,c

 
    

Regulator

Pyoverdine synthesis

Transport periplasma

Maturation periplasma

Secretion

Entire cluster

  
    

pvdS

pvdL pvdI pvdJ pvdD pvdH pvdA

pvdE

pvdQ pvdO pvdN pvdM pvdP

opmQ pvdR pvdT

   

soil a

s3a18

Residual non-producer

2.8

1

5

1

5

3

15

Lys-Orn-Gly-Gly-Thr-Ser-Orn

 

s3a10

Stimulating producer

127

1

5

1

5

3

15

Ser-Orn-Gly-Gly-Thr-Gly-X

 

s3a12

Non-stimulating producer

64

1

6

1

5

3

16

Lys-Asp-Gly-Thr-Gly-Orn

soil b

s3b5

Residual non-producer

1.4

1

6

1

5

3

16

Ser-Lys-X-Thr-Ser-Orn

 

s3b6

Stimulating producer

20

1

5

1

5

3

15

Lys-Orn-Gly-Gly-Thr-Ser-Orn

 

s3b2

Non-stimulating producer

42

1

7

1

5

3

17

Gly-Orn-Gly-Gly-Ser-Gly-Asp-Thr

 

soil f

s3f7

Residual non-producer

2.0

1

6

1

5

3

16

Ser-Lys-X-Thr-Ser-Orn

 

s3f10

Stimulating producer

97

1

5

1

5

3

15

Ser-Orn-Gly-Gly-Thr-Gly-X

 

s3f19

Non-stimulating producer

120

1

7

1

5

3

17

Asp-Lys-Asp-Ser-Thr-Gly-Thr-Lys-X

 

soil h

s3h17

Complete non-producer

0.02

1

1

2

N/A

 
 

s3h14

Stimulating producer

46

1

5

1

5

3

15

Gly-Lys-Thr-Ser-X-Orn

 

s3h9

Non-stimulating producer

77

1

6

1

5

3

16

Lys-Asp-Gly-Thr-Gly-Orn

pond A

3A5

Complete non-producer

0.001

1

1

1

1

4

N/A

 
 

3A18

Stimulating producer

139

1

7

1

5

3

17

Lys-Asp-Thr-Thr-Gly-Asp-Ser

 
 

3A7

Non-stimulating producer

90

1

5

1

5

3

15

Gly-Lys-Thr-Ser-X-Orn-Thr-Thr

pond E

3E20

Complete non-producer

0.01

1

1

3

5

N/A

 
 

3E19

Stimulating producer

51

1

6

1

5

3

16

Lys-Orn-Gly-Gln-Gly-Ser-Orn

 
 

3E13

Non-stimulating producer

61

1

7

1

5

3

17

Asp-Thr-Gly-Asp-Gln-Gln-Gly

 

pond F

3F3

Residual non-producer

3.1

1

7

1

4

3

16

Asp-Lys-Orn-Thr-Gly-Ser-Ser-Orn

 
 

3F6

Stimulating producer

63

1

6

1

5

3

16

Lys-Asp-Gly Thr-Gly-Orn

 

3F5

Non-stimulating producer

48

1

7

1

5

3

17

Gly-X-X-Asp-X-Orn

 

pond H

3H3

Complete non-producer

0.01

1

1

1

1

3

7

N/A

 
 

3H7

Stimulating producer

72

1

6

1

5

3

16

Lys-Asp-Gly-Thr-Gly-Orn

 

3H9

Non-stimulating producer

108

1

6

1

5

3

16

Ser-Lys-Thr-Ser-X-Orn-Thr-Thr-X

 
  1. aPyoverdine production (in percentage) was measured relative to the reference strains (Supplementary Table 1)
  2. bX = unknown amino acid (no significant hit with prediction software)
  3. cSymbols depict pyoverdines with identical peptide backbone composition