Fig. 5

siRNA knockdown of ARID5B alters immune/inflammatory response and lipid metabolism genes. After 3 h of LPS treatment (100 ng ml⁻¹) of THP1-monocyte ARID5B knockdown samples compared with control samples (scrambled siRNA), a the transcriptome (N = 8 per group) were enriched with the inflammatory response genes including the listed bio-functions and canonical pathways (enrichment FDR < 0.05 from IPA); proportion of genes upregulated shown as red, downregulated shown as blue. b Relative IL-1A levels (mean ± SD) of mRNA expression in cells (n = 3 per group, by RT-PCR) and protein expression in culture media (n = 3 per group, by ELISA) decreased. c Cell migration (mean ± SD, n = 10 and 4 per group in experiment 1 and 2, respectively), and phagocytosis (mean ± SD, n = 8, 4, and 4 per group in experiment 1, 2, and 3, respectively) were inhibited