Fig. 2
From: The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan
Long-lived RPKO strains show defective ribosome assembly and reduced translation. a and b Polysome profiles of a long-lived and b short-lived RPKO strains in relation to the wild-type strain show that the former have a lower 60S-to-40S ratio, and are characterized by the presence of half-mers. c Flow cytometry readout for nascent protein synthesis by Click-iT HPG in the different strains and conditions. d Quantification of global translation in RPKOs with respect to the wild-type strain. Error bars represent s.d. across three different biological replicates. **p < 0.01, ***p < 0.001. P-values were calculated using two-tailed Student’s t-test
