Fig. 7
From: ATRX is a regulator of therapy induced senescence in human cells
Stabilizing HRAS expression prevents the transition from quiescence to senescence. a–h Wild-type HRAS (HRASwt) or a vector control (vect) was stably expressed in LS8817. After 4 days of selection with puromycin, cells were treated with 1 μM of PD0332991 for seven additional days (7D PD) and compared to cycling (CTRL) cells and analyzed for the following: HRAS mRNA levels measured by qPCR (a), growth arrest by BrdU incorporation (b) and cyclin A immunoblot (c), SA-β-gal (d), HP1γ SAHF (e), number of ATRX foci on an individual basis (f), induction of four SASP factors as measured by qPCR (g), and ability to return to cell cycle once PD0332991 once the dug was removed (h). Representative SAHF images are shown below panel e. Colony formation in panel h was not quantified as individual colonies could not be distinguished. Individual experiments are presented and all data are represented as mean ± SEM from three independent fields of view. Asrterisk indicates p < 0.05 using a two-sided Student’s t-test. All images were taken at the same magnification. The scale bar is 20 microns. See also Supplementary Fig. 10 for uncropped blots
