Fig. 4
From: Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
Time course analysis of GP2 expression in H1 hESCs-derived PP and PH cultures. Flow cytometry analysis of undifferentiated H1 hESCs and PP and PH cultures from day 9 to 13 of differentiation. Cells were stained with anti-GP2 or anti-CD142. a Average percentage of cells expressing GP2 N = 11 (hESC), N = 4 (D9-12), N = 9 (D13) or CD142 N = 5 (hESC), N = 3 (D9-112), N = 4 (D13). Error bars indicate s.e.m. b Representative flow cytometry plots. c qPCR analysis of PDX1, NKX6-1, GP2, and CD142 from hESC to day 13. Expression levels normalized to TBP, and relative to adult pancreas (not shown). GP2 relative normalized expression ×10−5. N = 3 for hESC, d3, d6, and N = 4 for d7-13, error bars indicate s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test. d Representative flow cytometry analysis of day 13 cultures. Cells were stained for GP2 and PDX1 or NKX6-1 and IgG control antibodies. The different GP2/PDX1 and GP2/NKX6-1 fractions are identified by purple, blue, red, and green boxes. e Quantification of the GP2/PDX1 and GP2/NKX6-1 fractions, as measured by flow cytometry analysis at day 13, N = 5, error bars indicate s.e.m. ****p < 0.0001, One-way ANOVA. Abbreviations: D9, day 9; GP2, pancreatic secretory granule membrane major glycoprotein 2; CD142, tissue factor; NKX6-1, NK6 homeobox 1; OCT4, octamer-binding transcription factor 4; SOX17, SRY (sex determining region Y)-box 17; FOXA2, forkhead box A2; PDX1, pancreatic and duodenal homeobox 1; NGN3, neurogenin 3
