Fig. 5
From: Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
GP2 and CD142 comparative profiles. a–j Flow cytometry analyses of H1-derived day 13 PP cultures obtained using 10 mM nicotinamide (a–e, optimal differentiation) and 3.3 mM nicotinamide (f–j, suboptimal differentiation) during stage 4 of differentiation. Cells were stained with anti-GP2 and anti-CD142 and then fixed and stained for the intracellular markers PDX1 and NKX6-1. IgG controls are shown on the left of each panel. Green boxes highlight the low percentage of GP2+/PDX− cells (c–h), red box highlights the presence of CD142+PDX1− cells (j). Abbreviations: GP2, pancreatic secretory granule membrane major glycoprotein 2; CD142, tissue factor; NKX6-1, NK6 homeobox 1; PDX1, pancreatic, and duodenal homeobox 1
