Fig. 6

FAM46C KO 12-week-old mice display reduced blood hemoglobin levels and activated primary B lymphocytes proliferate faster. a–h Hematologic parameters of FAM46C KO and control animals: a Blood hemoglobin concentration (WT, n = 8; FAM46C KO, n = 6); b red blood cells count (RBC) (WT, n = 7; FAM46C KO, n = 7); c mean corpuscular volume (MCV) (WT, n = 10; FAM46C KO, n = 10); d mean corpuscular hemoglobin (MCH) (WT, n = 10; FAM46C KO, n = 10); e mean corpuscular hemoglobin concentration (MCHC) (WT, n = 10; FAM46C KO, n = 10); f blood platelets (WT, n = 9; FAM46C KO, n = 9); g Hematocrit (WT, n = 8; FAM46C KO, n = 8); h bone marrow (BM) plasmocytes (WT, n = 12; FAM46C KO, n = 12). P-values were calculated using two-way ANOVA tests (**P < 0.01, ****P < 0.0001). i Analysis of the proliferation rate of activated primary B cells. B lymphocytes isolated from FAM46C KO mice and matching WT controls were stained with CFSE and in vitro activated using IL4 and LPS. Cell divisions were monitored by levels of CFSE dilution. One representative experiment of 2 is presented. For each experiment splenic B lymphocytes from three individuals have been pulled, for both WT and mutant mice