Fig. 7

Global analysis of FAM46C substrates in MM cells. a, b MAplots of total RNA sequencing results representing differential expression analysis of FAM46C^WT-GFP and FAM46C^mut-GFP-transduced MM cell lines. SKMM1 (a) and H929 (b) show transcriptome wide moderate deregulation of gene expression. Statistically significant values (FDR < 1%) are shown in red. The 396 transcripts constituting the common part for SKMM1 and H929 MM cell lines are marked with green circles. c Venn diagram demonstrating the overlap between upregulated transcripts in SKMM1 and H929 MM cell lines. d Functional GO terms annotation clustering of significantly upregulated transcripts in both MM cell lines. e, f Histogram representing the distribution of FAM46C-dependent polyadenylation rations monitored in fraction #5 (e) and #6 (f) in SKMM1 cell lines. Modeled normal distribution is fitted as a red line to emphasize the outlying population of FAM46C polyadenylated transcripts. g Venn diagram demonstrating the overlap between transcripts shifting to both long poly(A) fractions in FAM46^WT overexpressing cells mutually in SKMM1 and H929 MM cell lines. h 396 transcripts constituting the common part for SKMM1 and H929 MM cell lines were used in DAVID Functional Annotation Clustering Analysis. i The same set of transcripts was used to characterize the 3′-UTRs and total mRNA lengths showing a bias towards shorter species in either