Fig. 8

FAM46C expression results in polyadenylation of selected mRNAs in MM cells. a Northern blot analysis of SSR4, CD320, and FTL transcripts from H929 (lanes 1–3), SKMM1 (lanes 4–6), Raji (lanes 7–9) and HL60 (lanes 10–12) cells transduced with GFP (lanes 1, 4, 7, 10), FAM46C^WT-GFP (lanes 2, 5, 8, 11), and FAM46C^mut-GFP (lanes 3, 6, 9, 12). Asterisks indicate cross-hybridization signals. b The SSR4 transcript is extensively polyadenylated by FAM46C in MM cells. High-resolution northern blot analysis of SSR4 transcripts from SKMM1 (lanes 1–6) and H929 (lanes 7–12) transduced with GFP (lanes 1, 2, 7, 8), FAM46C^WT-GFP (lanes 3, 4, 9, 10), and FAM46C^mut-GFP (lanes 5, 6, 11, 12) after RNase H treatment (lanes 1, 3, 5, 7, 9, 11) to remove the poly(A) tail in presence of oligo(dT)₂₅. Control reactions were carried out in the presence of oligo(dT) without RNase H (lanes 2, 4, 6, 8, 10, 12). Kinetics of polyadenylation of SSR4, NAPSA, FTL, and CD320 transcripts over the time course of FAM46C expression. c High- and low-resolution northern blot analysis of SSR4, NAPSA, GAPDH, and RN7 transcripts from H929 (lanes 1–7) and SKMM1 cells (lanes 8–14) transduced with FAM46C^WT-GFP up to 24 h. d High-resolution northern blot analysis of SSR4 and FTL transcripts from H929 (lanes 1–8) and SKMM1 cells (lanes 9–16) transduced with FAM46C^WT-GFP (lanes 1–4, 9–12) and FAM46C^mut-GFP (lanes 5–8, 13–16) up to 72 h. e Northern blot analysis of NAPSA and CD320 transcripts from H929 (lanes 1–4) and SKMM1 cells (lanes 5–8) transduced with FAM46C^WT-GFP up to 72 h