Fig. 9

FAM46C interacts with BCCIPβ and PABPC1 in human cells. a Visualizations of Co-IP-MS experiments using GFP-tagged FAM46C expressed in myeloma cells H929 (shown in blue) and SKMM1 (shown in red). Estimated quantities of identified proteins were calculated using the label-free quantification (LFQ) algorithm and are represented as dot–plot graphs. Protein abundance was calculated as LFQ intensity of the protein signal divided by its molecular weight and is shown on the x axis on a logarithmic scale. Specificity was defined as the ratio of protein LFQ intensity measured in the bait Co-IP to the background level (protein LFQ intensity in a sample) and is shown on the y axis on a logarithmic scale. b, c Western-blot detection of BCCIPβ and PABPC1 co-immunoprecipitated with: b FAM46C^WT-GFP and FAM46C^mut-GFP from stable HEK293 cell lines; c FAM46C^WT-GFP from transduced SKMM1 and H929 MM cell lines. Purification was performed at low (lanes 1–3) and high (lanes 4–6) ionic strengths. GFP-tagged FAM46C was detected with α-GFP antibodies. Please note that the lack of the GFP signal in the GFP only control (lanes 1 and 4) is because the blot was cutoff to avoid very strong GFP signal, which could influence the readout