Fig. 2 | Nature Communications

Fig. 2

From: A general reaction mechanism for carbapenem hydrolysis by mononuclear and binuclear metallo-β-lactamases

Fig. 2

Electronic absorption spectra of imipenem hydrolysis catalysed by mononuclear MβLs. a Sequence of difference spectra upon the reaction of 91 μM imipenem and 51.4 μM Zn(II)-GOB-18. The reaction progresses from yellow to green spectra. The time interval covers up to 50 s. b Sequence of difference spectra upon the reaction of 3.5 mM imipenem and 430 μM mono-Co(II)-GOB-18. The reaction progresses from blue to pink spectra. The time interval covers from 14.7 to 26.6 s. The inset shows a magnification of the 450–700 nm region. c Sequence of difference spectra upon the reaction of 3 mM imipenem and 350 μM mono-Zn(II)-Sfh-I. The reaction progresses from red to grey spectra. The time interval covers up to 50 s

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