Fig. 6
From: Caloric restriction delays age-related methylation drift
Expression of genes showing age-related methylation in mouse liver. a Gene expression in liver measured by quantitative RT-PCR. Values were normalized to the expression of Gapdh. The expression data for each gene were Z-score transformed. Each dot corresponds to one individual (AL young, n = 6, AL old, n = 11, CR old, n = 12). The values are presented as means of triplicate analyses. Z-scores were averaged for age-related hypermethylated and hypomethylated genes (bar graph, right side). White, black, and red solid bars represent the expression level of genes in AL young, AL old, and CR old samples, respectively. Bars represent standard errors. p-values were obtained using the unpaired t-test with Welch’s correction. b Unsupervised hierarchical cluster analysis of gene expression data. Expression values were Z-score transformed. Green boxes indicate lower expression than the mean expression and red boxes depict higher expression. (Red; high expression, green; low expression.) The dendrogram is shown at the right of the figure. The color codes of caloric status and age are shown on the bottom-right
