Fig. 7

CD137 expression on blood CD8⁺ T cells predicts sensitivity to the combination of anti-CTLA-4+ anti-PD-1 Ab. a, d Display of the Wilcoxon rank-sum test p-values vs. the log transformed ratio between responders (R) and non-responders (NR) to anti-CTLA-4 + anti-PD-1 Abs in the blood a and tumor d in the ex vivo mLN assay. Each dot represents one marker; selected biomarkers are shown in red while biomarkers with low level of expression are shown in gray. b−f Expression levels of CD137 in blood b, c and tumor bed e, f of CD4⁺ b, e and CD8⁺ T c, f cells, respectively, in patient lesions responding (R) or not (NR) in the ex vivo mLN assay anti-CTLA-4 + anti-PD-1 mAbs stimulatory condition. Each dot represents one patient. The absolute numbers of patients are indicated in both groups in parentheses. Graphs were analyzed by Wilcoxon rank-sum test. g, h Prospective analysis of CD137 expression on T cells prior to enrollment in a Phase II trial of high risk stage IIIc/IV MMel patients. Distributions of CD137 expression on blood CD8⁺ T cells at diagnosis prior to PD-1/CTLA-4 co-blockade g or PD-1 blockade h across patient groups stratified based on progression (relapse or no evidence of disease [NED]) with a median follow-up of 13 months post enrollment in adjuvant therapy for stage III/IV resected MMel. Each dot represents the flow cytometry analysis value of CD137 expression compared with an isotype control mAb prior to adjuvant therapy. Reported p-values are obtained from the Wilcoxon rank-sum test. Box and whisker plots are represented from the corresponding distribution b, c, e−h