Fig. 1
Identification of the chimeric HIV/BACH2 and HIV/STAT5B transcripts in patients under cART. a, b Scheme of the RT-PCR strategy devised to amplify putative chimeric HIV/STAT5B (a) or HIV/BACH2 (b) transcripts; white and gray boxes represent non-coding and coding exons (Ex), respectively. The exon numbers and the position of the first ATG codon are indicated. The integrated provirus, the LTR, and the 5′ major viral SD site are depicted in black. The arrows represent the position of the primer pairs used for RT-PCR. The black and gray bars indicate the amplified cDNA sequence from the provirus and host genome, respectively. The dashed lines indicate the splicing events. At the bottom of each panel, representative examples of the sequences of the RT-PCR products are shown. c, d Report agarose gel electrophoresis of RT-PCR products of either HIV/STAT5B or HIV/BACH2 obtained from PBMC of HIV-1-infected patients using the primers indicated in a, b; the amplicon size is ~500 bp (M molecular size markers). For each sample, RT-PCR for GAPDH was also performed as positive control of amplification (data not shown)
