Fig. 6
Effects of the forced expression of STAT5B and BACH2 in Treg cells. a Experimental strategy. Treg cells were purified from PBMC of HDs, were activated by anti-CD3/CD28 Treg expander beads for 24 h, transduced with the indicated LVs, and cultured for 10 days in presence of Treg expander beads and IL-2 (100 U/ml). b Inhibition of proliferation (analyzed by flow cytometry for e-Fluor dilution and indicated as % Suppression vs. responder alone) of anti-CD3/CD28 activated allogeneic Responder (R) cells cultured in presence of different doses (Ratio R:S were 1:1, 1:05, and 1:0.25) of Suppressive cells (S). c, d Percentage of Orange+ Treg cells expressing STAT5B (c) or BACH2 (d) measured over time and cultured alone (indicated as STAT5B and BACH2) or in presence of Treg cells transduced with the control LV (c) (Ratio GFP-expressing and STAT5B-expressing cells is 1:1, 1:05, and 1:025, the same for GFP-expressing and BACH2-expressing cells). Significance was determined by performing two-way ANOVA with Bonferroni correction on Log ODD transformed % values (*p < 0.05; **p < 0.01). Data are represented as Mean ± SEM, and for all panels N = 4
