Fig. 1

NFATc1 controls the re-organization of cytoskeleton and polarization of cytosolic organelles. a Spreading of WT and Nfatc1 ^−/− (Nfatc1 ^f/f x CD4-cre mice) CTLs on αCD3/CD28-coated glass slides within 5 min. Scanning electron microscopy. LP, lamellipodium. b Quantification of data of spreading (above) and F-actin ring formation (below) of CTLs activated for 5 min (t-test, unpaired, p-value < 0.0001). Left; each dot/triangle corresponds to one cell. Right; mean of three independent experiments. c Formation of F-actin rings in WT and Nfatc1 ^−/− CTLs upon αCD3/CD28 activation within 5 min. TIRF microscopy. d Appearance of MTOC-tubulin near the IS of CTLs upon activation for 5 min. TIRF microscopy. e Organization of F-actin and MTOC-tubulin in CTLs upon activation for 20 min. Confocal microscopy. For scale bar see d. f Repositioning of mitochondria and lytic granules to the IS in living WT but not Nfatc1 ^−/− CTLs. Left; analysis by confocal microscopy; overlay of bright field and merged fluorescence images of lysosomes (red) and mitochondria (green). The dark dots correspond to beads coated with αCD3/CD28. Right; quantification by epifluorescence microscopy. The column presentation compiles the data of more than 15 cells from 3 mice each