Fig. 4

Transcriptome assays of CD8⁺ T cells by next generation sequencing. a Heat map of 561 genes whose expression changed twofold in either Nfatc1 ^−/− (Nfatc1 ^f/f x CD4-cre mice) or Nfatc2 ^−/− aCD8⁺ Ts and CTLs. b Number of genes whose expression was changed 2.5-fold in either aCD8⁺ Ts, CTL− or CTL + cells. Genes whose expression was specifically changed in Nfatc1 ^−/− cells are shown in blue, those changed in Nfatc2 ^−/− cells in orange, and those changed in both in gray. c Scatterplots of NFATc1- and NFATc2-dependent genes relative to Log₂WT expression in aCD8⁺ Ts (upper panel) and CTL + cells (lower panel). d Functional annotation of genes changed 2.5-fold in RNA levels in NFATc1-deficient aCD8⁺ Ts and CTL + cells according to the PANTHER pathway analysis⁶⁹