Fig. 6

NFATc1 ablation affects the proliferation and differentiation of CD8⁺ T cells. a CFSE labeling of aCD8⁺ T cells stimulated for 3 d by plate-bound αCD3/CD28 (0.05/2 μg/ml), and of CTLs which were stimulated by αCD3/CD28 (5/2 μg/ml) for 3 d followed by IL-2 for 5 d. b Heat map of selected genes controlling the proliferation of Nfatc1 ^−/− (Nfatc1 ^f/f x CD4-cre mice) aCD8⁺ Ts. c Heat map of selected genes affecting the fate of CD8⁺ Ts. d Real-time PCR assays of Tbx21 and Eomes RNA expression in CTL + cells, normalized by Actb and relative to naïve CD8⁺ T cells. e Flow cytometry of intracellular T-bet and Eomes expression in CTL + cells. f ChIP seq assay of NFATc1/A-Bio and NFATc2¹⁹ binding to the Tbx21 gene in CTL + cells. g Real-time PCR assays of Ccr7, IL7r, Itgae and Irf4 RNA expression in CTLs, normalized by Actβ and relative to naïve CD8⁺ T cells. Data from five PCR assays are shown as means ± SEM. Unpaired Student’s t-test was used for statistics