Fig. 1
Interaction of CUG-BP2 RRM3 with AU-rich RNAs. a Schematic representation of full-length CUG-BP2. b Mapping of the combined chemical shift perturbations (ΔCS) observed on the backbone NH signals of RRM3 upon addition of saturating amounts of 5′-AUUUAAUU-3′ (1:1.33, open bars) and 5′-UUUAA-3′ (1:3, blue bars). c Overlaid expansions of the 1H–15N 2D-HSQCs of RRM3 alone (blue) and in the presence of saturating amounts of 5′-AUUUAAUU-3′ (red) or 5′-UUUAA-3′ (green). d Titration curves of CUG-BP2 RRM3 NH signals with 5′-AUUUAAUU-3′ (top) and 5′-UUUAA-3′ (bottom), respectively at 25 °C
