Fig. 6 | Nature Communications

Fig. 6

From: Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination

Fig. 6

Translation inhibition of COX-2 mRNA by CUG-BP2. a CUG-BP2 constructs. The flag-tag is depicted as a green bar and the RRMs as rounded rectangles. b Luciferase reporter gene. The coding sequence of the renilla luciferase is fused to the first sixty nucleotides of COX-2 mRNA 3′-UTR. c Luciferase reporter assay. Upper panel: normalized renilla/firefly luciferase activities for the different CUG-BP2 constructs. All the values are normalized to the negative control, which is the empty vector. Statistical significance was tested using the student test (a: significantly different from WT, p < 0.05; b: significantly different from control, p < 0.05). Error bars represent s.d. Lower panel: western blot

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