Fig. 2
From: Rapid and reversible epigenome editing by endogenous chromatin regulators
Reversible heterochromatin complex recruitment regulates Oct4 expression in mESCs. a Schematic representation of heterochromatin complex recruitment to the Oct4 locus in reporter Oct4-GFP mESCs. b Microscopy reveals loss of GFP expression in Oct4-GFP mESC colonies 5 days after RAP treatment to induce HP1cs recruitment. Arrows show examples of GFP-negative cells within Oct4-GFP ESC colonies. Scale bar represents 100 µm. c FACS analysis shows reversible repression of Oct4-GFP expression in mESCs. After 5 days of RAP to recruit HP1cs to the Oct4 locus, washout experiments using FK506 restore GFP expression levels after 5 days. Numbers represent % of GFP±cells d H3K9me3 ChIP analysis at the Oct4 locus (−739, −130, and +254 bp from transcriptional start site, TSS) 5 days post RAP treatment and 5 days after washout with FK506. n = 3; error bars s.e.m
