Fig. 5
From: Understanding CRY2 interactions for optical control of intracellular signaling
Optical control of Raf activation using CRY2 constructs with differential clustering capacities. a Illustrative scheme for light-induced activation of MAPK signaling pathway. b Light-activatable Raf1 with different CRY2 oligomerization propensities. c Cells expressing CRY2high-mCh-Raf1 yielded the highest level of pERK after light stimulation, while cells expression CRY2low-tdTom-Raf1 produced the lowest level of pERK after light illumination. Western blot analysis of phosphorylated ERK (pERK, Thr202 and Tyr204) was conduceted after blue light illumination at 0.2 mW cm−2 for 5 min. d The pERK value decreased from CRY2high-mCh-Raf1, CRY2wt-mCh-Raf1, CRY2low-mCh-Raf1 to CRY2low-tdTom-Raf1 after light stimulation. The pERK value in each transfection was averaged from two technical replicates from two independent experiments. The values were normalized against pERK from cells expressing CRY2wt-mCh-Raf1. Results are presented as means ± s.e.m. and analyzed using one-way ANOVA with Dunnett’s post hoc test. (*P < 0.05)
