Fig. 2
Analysis of spine types lost and percentages of spines formed and eliminated after axotomy. a Quantification of stubby, mushroom, and thin spine densities before axotomy, 24 h post-axotomy, 48 h post-axotomy, and in uninjured control chambers. Uninjured controls and axotomized samples were imaged beginning at 13 DIV (labeled “0 h” or “before”, respectively). Uninj. cntl: n = 20 dendrites; 5 neurons; 3 chambers over 3 experiments; #spines/TDL: 315/2698 µm (0 h), 388/2737 µm (24 h after). Axot.: n = 26 dendrites; 5 neurons; #spines/TDL: 405/3569 µm (before), 274/2998 µm (24 h after), and 229/2821 µm (48 h after). 3 chambers over 3 experiments. Repeated-measure two-way analysis of variance (ANOVA), Bonferroni post hoc test. b Representative images of dendritic segments from uninjured control and axotomized chambers at 0 h or before axotomy, respectively, and 24 h after. Asterisks indicate spines eliminated; arrows indicate formation of new spines. Scale bars, 5 µm. c, d Bar graphs represent percentage of spines eliminated c and newly formed d 24 h post-axotomy and in age-matched uninjured control chambers. Uninj. cntl: n = 28 dendrites; 6 neurons; #spines eliminated: 92; #spines formed: 177; TDL (0 h, 24 h after): 3147, 3148 µm. Axot: n = 35 dendrites; 6 neurons; #spines eliminated: 208; #spines formed: 75; TDL (before, 24 h after): 4290, 3865 µm. 3 chambers over 3 experiments. Unpaired two-tailed t-test. **p < 0.01, ****p < 0.0001. Error bars, s.e.m
