Fig. 7
Netrin-1 gene expression following axotomy in microfluidic cultures and in vivo following spinal cord hemi-transection Microarray analysis was performed on somatodendritic samples of uninjured control and 24 h post-axotomy chambers. Quality control data is presented in Supplementary Fig. 5. a Volcano plot showing differentially expressed RNAs that are significantly changed at 24 h post-axotomy (One-way between-subject ANOVA; n = 3 individual chambers each condition; Supplementary Table 2). b Microarray expression levels for Ntn1 within microfluidic chambers (left) and for housekeeping genes Odc1 and GAPDH (right). One-way between-subject ANOVA. c Microarray expression levels for Ntn1 (left) and Odc1 and GAPDH (right) in cortical layers V/VI following hemi-transection at thoracic level 8. n = 4 animals per group. Ntn1 levels are significantly reduced by 7 days following injury. Two-way ANOVA, Sidak’s multiple comparisons test. *p < 0.05. Error bars, s.e.m
