Fig. 6

VEGFR2 mediates POFUT1 function for coronary artery formation. a Survival curve of Pofut1 ^cKO and Pofut1;Vegfr2 ^DKO mice. b Gross view of P60 hearts. c Heart/body ratio of P60 mice. #p < 0.01 between control and Pofut1 ^cKO, *p < 0.01 between Pofut1 ^cKO and Pofut1;Vegfr2 ^DKO. Echocardiography shows that cardiac function of 2-month Pofut1;Vegfr2 ^DKO mice are restored. #,*p < 0.001. EDV/ESV, end diastolic/end systolic volumes; EF/FS, ejection fraction/fractional shortening. d Cardiac histology shows no sign of myocardial infarction in P60 Pofut1;Vegfr2 ^DKO hearts. e Quantification of Sirius Red staining. #p < 0.01 between control and Pofut1 ^cKO, *p < 0.01 between Pofut1 ^cKO and Pofut1;Vegfr2 ^DKO. ∆ p < 0.01 between outer and inner. f, g PECAM1 staining shows restored coronary network in E16.5 Pofut1;Vegfr2 ^DKO hearts. h, i VEGFR3/EdU co-staining shows reduced proliferation of angiogenic cells (arrowheads) to normal levels in P60 Pofut1;Vegfr2 ^DKO hearts. #,*p < 0.05. j, l VEGFR3 staining shows a restored number and distribution of angiogenic cells in E16.5 Pofut1;Vegfr2 ^DKO hearts. Mean ± SD; #,*p < 0.001. k, l PECAM1 staining shows a restored number of tip-like cells (arrowheads) in E16.5 Pofut1;Vegfr2 ^DKO hearts. Mean ± SD; #,*p < 0.001