Fig. 3

Conditional deletion of Oxtr impairs the survival of newly generated DGCs. a Schematic representation of the experimental designs for comparing the proliferation of newly generated DGCs in WT and Oxtr ^−/− mice. Mice were given a single injection of BrdU (50 mg/kg) and were killed 2 h after BrdU injection. b Schematic representation of the experimental designs for comparing the survival of newly generated DGCs in WT and Oxtr ^−/− mice. Mice were injected six times intraperitoneally with BrdU (50 mg/kg) at 12 h intervals and were killed 14 and 28 days after the last BrdU injection. c Representative immunofluorescence images of hippocampal sections from WT and Oxtr ^−/− mice double or triple stained for BrdU (green), Ki67 (red), DCX (red), and NeuN (blue) after BrdU injection. Scale bar, 100 µm. d Quantification of the total number of BrdU⁺ cells in the DG of WT and Oxtr ^−/− mice at 2 h after BrdU injection (n = 4 mice per genotype, unpaired two-tailed Student’s t test). e Quantification of the total number of Ki67⁺ cells in the DG of WT and Oxtr ^−/− mice at 2 h after BrdU injection (n = 5 mice per genotype, unpaired two-tailed Student’s t test). f Quantification of the total number of BrdU⁺ cells in the DG of WT and Oxtr ^−/− mice at 14 and 28 days after BrdU injection (n = 5 mice for each group; ***P < 0.001, unpaired two-tailed Student’s t test). g Quantification of the total number of DCX⁺ cells in the DG of WT and Oxtr ^−/− mice at 14 days after the last BrdU injection (n = 5 mice per genotype; ***P < 0.001, unpaired two-tailed Student’s t test). Data represent the mean ± s.e.m