Fig. 4

Conditional deletion of Oxtr delays the morphological maturation of newly generated DGCs. a Confocal three-dimensional reconstruction of dendrites of EGFP⁺ DGCs from WT and Oxtr ^−/− mice at 14 dpi. Scale bar, 50 µm. b, c Summary bar graphs depicting b the total dendritic length and c branch number of EGFP⁺ DGCs from WT and Oxtr ^−/− mice at 14 dpi (n = 11 neurons from 4 mice per genotype; **P < 0.01, ***P < 0.001, unpaired two-tailed Student’s t test). d Sholl analysis of dendritic complexity of EGFP⁺ DGCs from WT and Oxtr ^−/− mice at 14 dpi (n = 11 neurons from 4 mice per genotype; *p < 0.05, **P < 0.01, two-way ANOVA with Bonferroni’s post hoc test). e Representative confocal images showing EGFP⁺ DGCs from WT and Oxtr ^−/− mice at 28 dpi. Scale bar, 50 µm. f, g Summary bar graphs depicting f the total dendritic length and g branch number of EGFP⁺ DGCs from WT and Oxtr ^−/− mice at 28 dpi (n = 11 neurons from 5 mice per genotype; unpaired two-tailed Student’s t test). h Sholl analysis of dendritic complexity of EGFP⁺ DGCs from WT and Oxtr ^−/− mice at 28 dpi (n = 11 neurons from 5 mice per genotype; two-way ANOVA). i Top panel, schematic representations of the retroviral vectors used for in vivo genetic manipulation under the control of the Ubi-1 promoter. Bottom panels, representative confocal images showing Retro-EGFP⁺ and Retro-Cre-2A-EGFP⁺ DGCs in Oxtr ^f/f mice at 14 dpi. Scale bar, 50 µm. j Summary bar graphs depicting the total dendritic length of Retro-EGFP⁺ and Retro-Cre-2A-EGFP⁺ DGCs in Oxtr ^f/f mice at 14 dpi (n = 5 neurons from 3 mice for each group, unpaired two-tailed Student’s t test). k Sholl analysis of dendritic complexity of Retro-EGFP⁺ and Retro-Cre-2A-EGFP⁺ DGCs in Oxtr ^f/f mice at 14 dpi (n = 5 neurons from 3 mice for each group, two-way ANOVA). Data represent the mean ± s.e.m