Fig. 1

ILC2 are significantly increased in human APL. a Representative examples of flow cytometry analysis of innate lymphoid cells (ILC) in healthy donors (HD) and APL patients’ (APL) peripheral blood. HD were sex- and age-matched with APL patients, M:F 12:10 vs. 12:10, median age 45 vs. 43, min 27 vs. 23, max 72 vs. 77 years. b Frequencies (n = 22) and c cell numbers/µl (HD n = 20, APL n = 13) of total ILCs identified as Lin⁻CD127⁺ cells in lymphocytes. d Representative examples of flow cytometry analysis of innate lymphoid cell subsets in HD and APL patients’ (APL) peripheral blood. e Relative frequencies (n = 22) f cell numbers/µl (HD n = 20, APL n = 13) of ILC subsets among total ILCs. g Representative examples of flow cytometry analysis of ILC subsets in the bone marrow of healthy donors (HD BM) and APL patients (APL BM). h Relative frequencies of ILC subsets among total ILCs (HD n = 5, APL n = 11). i Representative examples of flow cytometry analysis of ILC subsets in paired APL BM and peripheral blood (APL PB) samples of APL patients. j Relative frequencies of ILC subsets among total ILCs (n = 11). k Frequency of Ki-67 expressing ILC2 in HD, APL BM and APL PB (n = 11). ILC subsets are defined as follow: ILC1: Lin⁻CD127⁺CRTH2⁻cKit⁻; ILC2: Lin⁻CD127⁺CRTH2⁺cKit^−/+; ILC3 NCR⁺: Lin⁻CD127⁺CRTH2⁻cKit⁺NKp46⁺; ILC3 NCR⁻: Lin⁻CD127⁺CRTH2⁻cKit⁺NKp46⁻. Error bars are s.e.m. Statistical analysis was performed using t test (a–f), Mann-Whitney test (h–j) and ANOVA test (k)