Fig. 4

Identification of transcriptional targets for ZNF516. a MCF-7 cells were transfected with Gal4-DBD or Gal4-ZNF516 plasmids and were treated with trichostatin A (TSA) or tranylcypromine. Forty-eight hours later, luciferase activity of Gal4-SV40-LUC was measured. Relative luciferase activity was calculated as firefly luciferase activity divided by renilla luciferase activity and shown relative to the control. Each bar represents the mean ± S.D. for triplicate experiments. P-values were determined by Student’s t-test. *P < 0.05. b Genomic distribution of ZNF516 and CtBP1 (published in ref. ⁴⁶) determined by ChIP-seq analysis in MCF-7 cells. Number of peaks in each cluster was indicated. c The Venn diagram of overlapping genes targeted by ZNF516 and CtBP1 in MCF-7 cells (left). The clustering of the 425 overlapping target genes of ZNF516/CtBP1 into biological process ontologies is shown (right). The details of the ChIP-seq experiments were provided in the Materials and Methods and results on the ontologies were provided in Supplementary Data 2. d The analysis of ZNF516-bound motifs and CtBP1-bound motifs using MEME suite. Top 10 motifs enriched at ZNF516 peaks were listed in Supplementary Fig. 2