Fig. 2

Pyrazinamide selection screens in vitro and during infection in mice. a Schematic diagram of the sequential in vitro pyrazinamide (PZA) selection screen. b Mean time-to-positivity (TTP; hours) of three biological replicates and standard deviations for each round of in vitro selection. c Mean and standard deviations of bacterial burdens (colony-forming units CFU) observed in the spleens of mice at day −3 (implantation; gray), day 0 (start of pyrazinamide treatment; gray) and after 21 and 42 days of pyrazinamide (PZA; black) or mock (brown) treatment. Five mice per time-point were analyzed