Fig. 1

Diversity of IgG1 memory B cells (MBC) in type 2 responses. a, b IgG1⁺ MBC subsets in spleen and mesenteric LN (mLN) of TBmc mice immunised with OVA-PEP1 (OVA-PEP1 immunisation), and of BALB/c mice infected with Nippostrongylus brasiliensis (N.brasiliensis infection) at 10 weeks after treatment. The samples in each group were pooled, pre-enriched by depletion of IgD⁺, CD138⁺ and CD3⁺ cells. a Frequency of GL7⁺ germinal centre (GC) cells and PDL2⁺ MBC among gated IgG1⁺ B220⁺ cells (left), and identification of CD73⁺CD80⁺ (DP), CD73^–CD80⁺ (SP) and CD73⁻CD80⁻ (DN) IgG1 MBC subsets (right). b Frequency of CD73⁺CD80⁺ (DP), CD73^–CD80⁺ (SP), CD73⁻CD80^– (DN) and CD73⁺CD80^– cells among gated PDL2⁺IgG1⁺ MBC from OVA-PEP1 immunised TBmc mice (top) or N.brasiliensis infected BALB/c mice (bottom). Data are mean ± SEM of 5 OVA-PEP1 immunisation experiments and six N.brasiliensis infection experiments