Fig. 4
High affinity PC are selected from pre-existent memory clones. The repertoires of VDJ sequences from donor IgG1 MBC subsets (from 10 weeks OVA-PEP1 immunised TBmc mice) and their IgG1 and IgE progenies in spleen and BM of recipient mice (2 weeks after transfer/immunisation), were used to investigate the precursor-progeny relationship. a In silico derived sublibrary of the top 20 (frequency descending) VDJ CDR3 aa sequences containing high affinity mutations (in red). b Radar charts show the presence and frequency of each of the top 20 high affinity CDR3 sequences in donor DP IgG1 MBC and in the progeny IgE (left) and IgG1 (right) libraries. a, b The data are from one representative mouse of three recipient mice. c Percentage of the progeny IgE and IgG1 nucleotide sequences (nt seq) encoding high affinity CDR3 that were found in the donor DP IgG1 MBC. M1– M3 indicate individual recipient mice
