Fig. 7
From: Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
Tumor cell-released Hsp70/90-expressing EVs are critical to the development of muscle wasting in mice. a Elevation of serum Hsp70/90 in LLC tumor-bearing mice is dependent on EV release. LLC cells transduced with lentivirus encoding Rab27a- and Rab27b-specific shRNA or empty vector were analyzed for knockdown effect by western blotting (top). Mice were then implanted with Rab27-deficient or control LLC cells. In 21 days serum levels of EVs, Hsp70/90 were analyzed. b Rab27-deficient LLC tumor does not induce muscle catabolism in mice. Mice derived from a were analyzed for activity of the catabolic markers in TA. c Rab27-deficient LLC tumor does not induce muscle wasting in mice. Mice derived from a were analyzed for muscle wasting. Scale bar, 100 μm. Data (n = 6) were analyzed by analysis of variance or χ 2 analysis (for CSA). * denotes a difference (P < 0.05)
