Fig. 1
Transient vs. stable anchor-matrix bonds. Diffusion of 200 nm polyethylene glycol (PEG)-conjugated latex nanoparticles in biotinylated Matrigel® modified with neutravidin. a Representative traces of nanoparticles in Matrigel® with no added IgG (control), anti-PEG IgG (IgG), or biotinylated anti-PEG IgG (IgG-biotin) exhibiting effective diffusivities within one SEM of the ensemble average at a timescale of 1 s. b Distributions of the mean logarithms of individual particle effective diffusivities (D eff) at a timescale of 0.2667 s. Log (D eff) values to the left of the dashed line correspond to particles with displacements of less than 100 nm (i.e., roughly the particle diameter) within 0.2667 s. c Ensemble-averaged geometric mean square displacements (<MSD>) as a function of timescale, d mean D eff of all particles in each condition, and e fraction of mobile nanoparticles in Matrigel® treated with different IgG. N = 4 separately prepared slides/condition with 83–237 particles tracked per slide. Error bars represent SEM. *p < 0.05 compared to control in indicated comparisons. p values were calculated by repeated measures two-way ANOVA in c, with one-way ANOVA on log-transformed data in d, and with one-way ANOVA in e
