Fig. 3
From: Foxp3+ Tregs are recruited to the retina to repair pathological angiogenesis
Expanding Treg numbers reduced retinal vaso-obliteration in OIR. a Representative confocal images of flat mounted retina from C57BL/6J mice at postnatal day (P) 12 in phase I OIR and labeled with isolectin (green) to show blood vessels. Scale bar, 0.5 mm. b One quadrant (yellow box) from each retina has been enlarged. Scale bar, 0.25 mm. Retinas from room air control mice show normal vascularization, while retinas from OIR controls and OIR + anti-IL-2 mAb have vaso-obliterated blood vessels (asterisks) in the central retina. Mice treated with an IL-2/anti-IL-2 mAb complex or adoptively transferred (AT) Tregs have reduced retinal vaso-obliteration. Quantitation in phase I OIR at P12 c and phase II OIR at P18 d **P < 0.01 and ***P < 0.001 to OIR control groups. # P < 0.05 to OIR + AT Tregs (one-way ANOVA with Mann–Whitney U test). n = 9 mice/group with three litters examined. Values are mean ± s.e.m
